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The PBS was removed and cells were incubated for an additional 10 minutes at 37°C in PBS
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Clinically, H₂-enriched dialysis solutions correlate with reduced oxidative stress and inflammation, alongside improved patient-reported outcomes in end-stage renal disease.7,30,43,44,45,46,47 Recent technological developments, including real-time dialysate H₂ monitoring, may enhance clinical implementation,30,45 and preliminary work in peritoneal dialysis also suggests the preservation of membrane integrity.33,41 Given H₂ demonstrated renoprotective effects in chronic settings, next we explore its potential benefits in renal transplantation, which involves additional acute and chronic inflammatory challenges
